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Microscopes and Macroscopes using low NA objectives have to be characterized and calibrated for the different zooms. The field distortions present in this low NA objectives are significant and can distort the sample under investigation. We show how phase retrieval and wavefront sensing can be used to characterize them.
Uploaded by: praveenpankaj Added: 1 year ago
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Tags:
wavefront sensing
phase retrieval
low NA
Macroscope
fluorescence
widefield
confocal
aberrations
Bayeisan
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